Comprehensive imaging of gastroesophageal biopsy samples by spectrally encoded confocal microscopy

摘要: Background Spectrally encoded confocal microscopy (SECM) is a high-speed reflectance technique that has the potential to be used for acquiring comprehensive images of entire distal esophagus endoscopically with subcellular resolution. Objective The goal this study was demonstrate large-area SECM in upper GI tissues and determine whether contain microstructural information useful pathologic diagnosis. Design A feasibility study. Setting Gastrointestinal Unit, Massachusetts General Hospital. Patients Fifty biopsy samples from 36 patients undergoing routine EGD were imaged by SECM, their entirety, immediately after removal. Results microstructure seen similar histopathology. Gastric cardia mucosa clearly differentiated squamous mucosa. fundic/body type showed more tightly packed glands than gastric Fundic gland polyps cystically dilated lined cuboidal epithelium. presence intraepithelial eosinophils detected cells demonstrating characteristic bilobed nucleus. Specialized intestinal metaplasia identified columnar epithelium goblet cells. Barrett's (BE) dysplasia specialized loss nuclear polarity disorganized glandular architecture. Limitations Ex vivo, descriptive Conclusions Large-area gastroesophageal enabled visualization both architectural features various mucosal types corresponding histopathologic slides. These results suggest development an endoscopic probe merited.