Two rapid and simple enzyme immunoassays for human antibodies to Entamoeba histolytica.
作者： Tsue-Ming Lin , Charles M. Schubert , David J. Kiefer , Sandy Troy , Robert Cort
DOI: 10.1080/01971528908053243
关键词:
摘要: Two rapid and simple enzyme immunoassays (EIA) for antibodies to E. histolytica the protozoa causing ambiasis, are described. In dot EIA, a qualitative procedure, antigens were dried as small (3 mm in diameter) on thin white opaque polystyrene strip serum samples assayed undiluted. The assay required 3 incubation periods, 1 minutes each, was completed 9 minutes, with positive reaction revealed blue color (precipitate) antigen negative colorless. developed is stable permanent record. Microwell quantitative Microwells. also consisted periods of 15 50 minutes. results absorbance values normalized EIA units (EU). Both tests had good reproducibility, sensitivity specificity; highly correlated other serologic tests. Their reagents can be stored more than year. could suitable physicians' office laboratories, especially developing countries.
sci-hub.se 参考文章(16)
Seymour P. Halbert, Tsue-Ming Lin, Enzyme Immunoassay of Antibody Enzyme-Mediated Immunoassay. pp. 223- 239 ,(1985) , 10.1007/978-1-4684-5012-5_13
Enzyme-mediated immunoassay Plenum Press. ,(1985) , 10.1007/978-1-4684-5012-5
H. J. Bos, W. J. Schouten, B. F. J. Oostburg, H. Noordpool, M. Makbin, A seroepidemiological study of amebiasis in Surinam by the enzyme-linked immunosorbent assay (ELISA). American Journal of Tropical Medicine and Hygiene. ,vol. 29, pp. 358- 363 ,(1980) , 10.4269/AJTMH.1980.29.358
T M Lin, M W Chin-See, S P Halbert, J M Joseph, An enzyme immunoassay for immunoglobulin M antibodies to Toxoplasma gondii which is not affected by rheumatoid factor or immunoglobulin G antibodies. Journal of Clinical Microbiology. ,vol. 23, pp. 77- 82 ,(1986) , 10.1128/JCM.23.1.77-82.1986
J Chen, T M Lin, C M Schubert, S P Halbert, Treponemal antibody-absorbent enzyme immunoassay for syphilis. Journal of Clinical Microbiology. ,vol. 23, pp. 876- 880 ,(1986) , 10.1128/JCM.23.5.876-880.1986
J Yang, M T Kennedy, Evaluation of enzyme-linked immunosorbent assay for the serodiagnosis of amebiasis. Journal of Clinical Microbiology. ,vol. 10, pp. 778- 785 ,(1979) , 10.1128/JCM.10.6.778-785.1979
B.K. Van Weemen, A.H.W.M. Schuurs, Immunoassay using antigen-enzyme conjugates FEBS Letters. ,vol. 15, pp. 232- 236 ,(1971) , 10.1016/0014-5793(71)80319-8
Tsue Ming Lin, Monroe W. Chin-See, Seymour P. Halbert, The stability of prostatic acid phosphatase, as measured by a capture immunoenzyme assay Clinica Chimica Acta. ,vol. 138, pp. 73- 86 ,(1984) , 10.1016/0009-8981(84)90355-3
Aruna Tandon, Use of enzyme linked immunosorbent assay in intestinal and extra-intestinal amoebiasis (amoebic liver abscess). Transactions of The Royal Society of Tropical Medicine and Hygiene. ,vol. 75, pp. 574- 575 ,(1981) , 10.1016/0035-9203(81)90204-2
D. P. Hartmann, E. Ghadirian, E. Meerovitch, Enzyme-linked immunosorbent assay (ELISA) and indirect hemagglutination (IHA) test in the serodiagnosis of experimental hepatic amebiasis. Journal of Parasitology. ,vol. 66, pp. 344- 345 ,(1980) , 10.2307/3280833