Human eosinophils elaborate the lymphocyte chemoattractants. IL-16 (lymphocyte chemoattractant factor) and RANTES.

摘要: Eosinophils and CD4+ lymphocytes are preferentially recruited into sites of allergic inflammation. A role for eosinophils in the recruitment has not been defined. We studied capacity human to release chemoattractants T lymphocytes. Supernatants cultured contained chemoattractant activity lymphocytes, which was predominantly due IL-16 (lymphocyte factor) RANTES. With neutralizing Abs, eosinophil-derived lymphocyte chemotactic diminished by a mean (+/- SEM) 60 +/- 3% with polygonal anti-IL-16 Ab, 69 4% mAb, 48 anti-CD4 F(ab) (IL-16 receptor blockade), 40 anti-RANTES 88 5% combination mAbs. RANTES were detectable supernatants ELISA. constitutively expressed mRNA transcripts both reverse transcription-PCR preformed demonstrable ELISA cell lysates immunocytochemistry freshly isolated eosinophils. Thus, source two cytokines, RANTES, that as well These data indicate could contribute cytokines enhance additional populations