Systems and methods of droplet-based selection

作者: David A. Weitz , John Heyman

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摘要: The present invention generally relates to fluidic droplets, and techniques for screening or sorting such droplets. In some embodiments, the droplets may contain cells (e.g., hybridoma cells) that can secrete various species, as antibodies, example. one aspect, a plurality of containing is screened determine proteins, polypeptides, peptides, nucleic acids, like. For example, able species antibodies be se according certain embodiments invention. Examples include, instance, immortal hybridomas, non-immortal B-cells. blood encapsulated within produce determined. cases, expression secretion levels determined using signaling entities, determinable microparticles droplet. Other aspects relate kits involving methods promoting making use

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