Process for producing heterologous polypeptides

摘要: Processes for producing various heterologous polypeptides which when expressed are either incorrectly processed and hence asssociated with the surface of host cell or not to mature form. More specifically, processes production non-human carbonyl hydrolases in cells incapable enzymatically active endoprotease deficient extracellular disclosed. Such generally autoproteolytic maturation become associated expression hosts endoprotease. preparing hydrolase as part a fusion polypeptide also disclosed, well substantially free membrane they normally associated.