Identifying and mitigating bias in next-generation sequencing methods for chromatin biology
作者： Clifford A Meyer , X Shirley Liu , None
DOI: 10.1038/NRG3788
关键词:
摘要: Next-generation sequencing (NGS) technologies have been used in diverse ways to investigate various aspects of chromatin biology by identifying genomic loci that are bound transcription factors, occupied nucleosomes or accessible nuclease cleavage, physically interact with remote loci. However, reaching sound biological conclusions from such NGS enrichment profiles requires many potential biases be taken into account. In this Review, we discuss common which may introduced profiling data, approaches diagnose these and analytical techniques mitigate their effect.
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