A selective and rapid method for the quantification of captopril in human plasma using liquid chromatography/selected reaction monitoring mass spectrometry

摘要: A specific hyphenated high performance liquid chromatography-mass spectrometric (LC-MS/MS) assay was developed for the determination of captopril in plasma. The drug extracted from plasma using liquid-liquid extraction with a mixture diethylether:dichloromethane. After addition internal standard, samples were applied to prepacked C8 Waters Symmetry column. ion trap MS/MS detector equipped electrospray ionization (ESI) source operating positive mode. Drug accomplished monitoring at molecular m/z 218 and (daughter) 171.6. method human after administration 50 mg tablets healthy volunteers who have participated pharmacokinetic study. proved be precise by testing six different batches. Linearity established range concentrations 25-3000 ng/ml regression factor 0.9995. Intra-day accuracy ranged 90.16 96.18%, while intra-day precision 2.60 9.66% 75, 1440 2500 ng/ml. Inter-day 5.04 10.10%. This validated analysis successfully analyses single dose volunteers.