Interferon-γ Treatment Induces Granulomatous Tissue Reaction in a Case of Localized Cutaneous Leishmaniasis

摘要: Leishmania spp. suppress macrophage activity as part of their interaction with the immune system. Interferon-gamma (IFNgamma), a cytokine that participates in activation macrophages and killing intercellular parasites, induces healing leishmaniasis. We investigated sequence local systemic inflammatory cell parameters after IFNgamma therapy patient chronic, localized, cutaneous leishmaniasis caused by donovani. Histology, immunohistochemistry, polymerase chain reaction (PCR) for L. donovani, analysis T-cell receptor gene fragments from skin lesions well peripheral blood phenotyping were performed before, during, therapy. During therapy, epithelioid granulomas developed high number lesional human leukocyte antigen (HLA) DR+ macrophages, HLA-DR expression on monocytes increased to counts, indicating activation. Simultaneously, receptor-beta gene-specific PCR showed peak at 243 base pairs, clonal expansion Leishmania-reactive T lymphocytes. After detected minimal residual leishmanial DNA lesions, suggesting destruction parasites. In conclusion, compensates parasite-dependent major histocompatibility complex class II downregulation chronic