Some effects of glucose concentration and anoxia on glycolysis and metabolite concentrations in the perfused liver of fetal guinea pig.

摘要: Effects of glucose concentration and anoxia upon the metabolite concentrations rates glycolysis respiration have been investigated in perfused liver fetal guinea pig. In most cases were similar to those observed vivo. Between 50 days term there was a fall respiratory rate ATP fructose 1,6-diphosphate an increase glutamate, glycogen glucose. Reducing medium from 10 mM 1 or 0.1 depressed lactate production phosphorylated intermediates (except 6-phosphogluconate) 50-day fetus. This indicates glycolytic which is not accord with known kinetic properties hexokinase liver. Anoxia increased by, of, hexose phosphates ADP AMP liver, while ribulose 5-phosphate, some triose fell. These results are consistent activation glycolysis, particularly at phosphofructokinase reduction pentose phosphate pathway activity, 6-phosphogluconate dehydrogenase. The calculated cytosolic NAD+/NADH ratio for that measured vivo evidence presented suggest dihydroxyacetone phosphate/glycerol 3-phosphate gives better indication redox than lactate/pyruvate ratio. present observations indicate possibly pyruvate kinase control glyceraldehyde-3-phosphate dehydrogenase equilibrium