Inflamed synovial fluid induces a homeostatic response in bone marrow mononuclear cells in vitro: Implications for joint therapy

摘要: Synovial inflammation is a central feature of osteoarthritis (OA), elicited when local regulatory macrophages (M2-like) become overwhelmed, activating an inflammatory response (M1-like). Bone marrow mononuclear cells (BMNC) are source naive capable reducing joint and producing molecules essential for cartilage metabolism. This study investigated the BMNC to normal (SF) inflamed synovial fluid (ISF). Equine cultured in autologous SF or ISF (n = 8 horses) developed into macrophage-rich cultures with phenotypes similar native became more confluent (~100%) than (~25%). were neither M1- nor M2-like, but exhibited aspects both immune response, characterized by increasing counts IL-10+ macrophages, decreasing IL-1β concentrations progressively IL-10 IGF-1 concentrations. Changes marked suggest that homeostatic mechanisms preserved over time potentially favored progressive cell proliferation. Collectively, our data intra-articular could increase macrophage counts, potentiating macrophage- IL-10-associated homeostasis lost during progression OA, preserving production cytokines involved tissue repair (PGE2 , IL-10) generally impaired frequently used corticosteroids.