DNA interaction and anticancer properties of new peripheral phthalocyanines carrying tosylated 4-morpholinoaniline units

摘要: Abstract In this paper, tosylated 4-morpholinoaniline units fused peripherally tetra-substituted free-base (5), copper(II) (6), zinc(II) (7), cobalt(II) (8) and magnesium(II) (9) phthalocyanines (Scheme 1) were reported these new phthalocyanine conjugates characterized through Fourier Transform–Infrared (FT–IR) with ATR sampling accessory, Mass Spectra Analysis [Matrix Assisted Laser Desorption/Ionization–Time of Flight–Mass Spectral (MALDI–TOF–MS)] Ultraviolet–visible (UV–vis) (for all phthalocyanines), elemental analysis, as well 1H 13C NMR spectroscopic techniques [for the compounds (2), (4), (7) (9)]. The potential utilization peripheral (7–9) pharmaceutical agents in PDT applications (in oncology molecular biology) determined aspects pBR322 plasmid DNA cleavage on agarose gel electrophoresis. results showed that compound cleaved irradiation. Compound displayed hypochromism without any shift addition increasing concentrations ct-DNA Kb was calculated 2.45 ± (0.20) × 104 M−1. photochemical studies, ΦΔ value 0.11. cytotoxic/phototoxic properties which had best photocleavage effects among tested investigated using MTT assay toward human colorectal (HCT-116) cervical (HeLa) cancer cells. cell viabilities found to be 73 ± 1.6% 65 ± 5.5% at 100 µM