Time course involvement of matrix metalloproteinases in the vascular alterations of renovascular hypertension.

摘要: Abstract Increased vascular matrix metalloproteinases (MMPs) levels play a role in late phases of hypertensive remodeling. However, no previous study has examined the time course MMPs various two-kidney, one-clip hypertension (2K1C). We structural changes, collagen and elastin content, oxidative stress, levels/activities during development 2K1C hypertension. Plasma angiotensin converting enzyme (ACE) activity was measured to assess renin-angiotensin system activation. Sham or rats were studied after 2, 4, 6, 10 weeks Systolic blood pressure (SBP) monitored weekly. Morphometry changes aortic wall hematoxylin/eosin, orcein picrosirius red sections. Aortic NADPH superoxide production evaluated. gelatinolytic determined by situ zymography, MMP-2, MMP-14, tissue inhibitor (TIMP)-2 gelatin immunofluorescence immunohistochemistry. associated with increased ACE activity, which decreased normal 10 weeks. found content early phase hypertension, hypertrophy, MMP-2 MMP-14 (but not TIMP-2) levels, possibly as result oxidase stress. These results indicate that remodeling renovascular is an process increases activities, enhanced deposition Using antioxidants inhibitors may prevent alterations