Site-specific Uncoupling of Photosynthetic Phosphorylation in Spinach Chloroplasts

摘要: Abstract In spinach chloroplasts which have been shaken with n-heptane, photophosphorylation coupled to the reduction of nicotinamide adenine dinucleotide phosphate is inhibited about 95% while photoreduction NADP+ (and ferricyanide) stimulated as much 50%. Photophosphorylation catalyzed by phenazine methosulfate, however, only These data are interpreted indicate that there two sites in chloroplasts. Both utilized cyclic photophosphorylation, whereas one common both and noncyclic photophosphorylation. heptane-treated site functioning associated exclusively electron flow. Heptane treatment also has a differential effect upon adenosine triphosphatase activities Trypsin-activated Ca++-dependent ATPase not affected heptane treatment, Mg++-dependent activated light presence dithiothreitol 80 90%. After ability accumulate protons illumination 40 60% their synthesize ATP when transferred from acidic alkaline environment