Assessment of the Effects of 6 Standard Rodent Diets on Binge-Like and Voluntary Ethanol Consumption in Male C57BL/6J Mice.

摘要: Background In recent years, much attention has been given to the lack of reproducibility in biomedical research, particularly preclinical animal studies. This is a problem that also plagues alcohol research field, consistent consumption models use disorders. One often overlooked factor could affect maintenance diet used studies. Methods Herein, 2 well-established consumption, “drinking dark” (DID) procedure and continuous 2-bottle choice (C2BC) paradigm, were employed determine effects on ethanol (EtOH) consumption. Male C57BL/6J mice 1 6 standard rodent chow diets obtained from Purina LabDiet®, Inc. (Prolab® RMH 3000) or Harlan® Laboratories, (Teklad Diets T.2916, T.2918, T.2920X, T.7912, T.8940). A separate group animals test dietary EtOH pharmacokinetics behavioral measures following intraperitoneal (IP) injections various doses EtOH. Results Mice eating Harlan T.2916 (H2916) T.2920X (H2920) consumed significantly less exhibited lower blood concentrations (BECs) during DID; however, C2BC, maintained T.7912 (H7912) more had higher preference than other groups. levels did not stem changes pharmacokinetics, as administered IP showed no difference BECs. However, sensitive alcohol-induced locomotor activity an open-field task. No diet-dependent differences seen sedation measured with loss righting reflex. Conclusions Although these data do identify specific mechanism, together, they clearly show impacts It incumbent upon community consider importance describing nutritional information methods, which may help decrease interlaboratory issues.