Relaxin-3 and receptors in the human and rhesus brain and reproductive tissues

摘要: Evidence suggests that relaxin-3 may have biological functions in the reproductive and central nervous systems. To date, however, biodistribution has only been investigated mouse, rat, pig teleost fish. Characterizing gene structure, expression patterns, function non-human primates humans is critical to delineating its significance. Experiments were performed clone rhesus macaque orthologues of peptide hormone cognitive receptors (RXFP1 RXFP4). An investigation bioactivity RXFP1 binding properties was also performed. Next we sought investigate immunoreactivity human tissues. Immunohistofluorescence staining for brain, testis, prostate indicated predominant immunostaining ventral dorsal tegmental nuclei, interstitial space surrounding seminiferous tubules, prostatic stromal cells, respectively. Further, studies designed towards exploring functions, observed neuroprotective actions on neuronal cell cultures. Taken together, this study broadens significance as a involved both tissues primates.