Resolution of Holliday junctions in Escherichia coli: identification of the ruvC gene product as a 19-kilodalton protein.

摘要: The ruvC gene of Escherichia coli specifies a nuclease that resolves Holliday junction intermediates in genetic recombination (B. Connolly, C.A. Parsons, F.E. Benson, H.J. Dunderdale, G.J. Sharples, R.G. Lloyd, and S.C. West, Proc. Natl. Acad, Sci. USA 88:6063-6067, 1991). was located between aspS the ruvAB operon by DNA sequencing deletion analysis plasmids shown to encode protein 18,747 Da. Analysis flanking indicated is transcribed independently LexA-regulated not under direct SOS control. lies downstream an open reading frame, orf-33, for which migrates during sodium dodecyl sulfate-polyacrylamide gel electrophoresis as 33-kDa polypeptide. These two genes probably form operon. However, expression found be very poor relative orf-33. A double ribosomal frameshift these proposed possible reason low level RuvC. Two further frames unknown function were identified, one on either side orf-33-ruvC Images