Morphological Diversity of Mitochondria in Cultured Astrocyte, HeLa, COS7 Cells under High Voltage Electron Microscopy

作者: Hyun-Wook Kim , Seung Nam Park , Younghye Moon , Seung Hak Oh , Im Joo Rhyu

DOI: 10.9729/AM.2013.43.3.117

关键词:

摘要: Mitochondrion is an important intracellular organelle controlling energy production essential for cell survival. In addition, it closely related to cellular apoptosis and necrosis. Linear, branched, circular, ball-shaped mitochondria have been reported. Recent research suggests that mitochondrial morphology may reflect functional status of the cell. this study, we investigated density ratio each morphological categories in a few normal cultured cells; astrocyte, HeLa COS7 cells, which metabolic activities are different, with high voltage electron microscopy. The absolute number relative per unit area was largest astrocyte. But, proportion different shape similar among cells. These results shows numerical profiles but not activity line.

参考文章(18)
The role of astroglia in neuroprotection Dialogues in clinical neuroscience. ,vol. 11, pp. 281- 295 ,(2009) , 10.31887/DCNS.2009.11.3/MBELANGER
Cagla Eroglu, Ben A. Barres, Regulation of synaptic connectivity by glia Nature. ,vol. 468, pp. 223- 231 ,(2010) , 10.1038/NATURE09612
H.-H. Tsai, H. Li, L. C. Fuentealba, A. V. Molofsky, R. Taveira-Marques, H. Zhuang, A. Tenney, A. T. Murnen, S. P. J. Fancy, F. Merkle, N. Kessaris, A. Alvarez-Buylla, W. D. Richardson, D. H. Rowitch, Regional Astrocyte Allocation Regulates CNS Synaptogenesis and Repair Science. ,vol. 337, pp. 358- 362 ,(2012) , 10.1126/SCIENCE.1222381
GEORGE E. PALADE, AN ELECTRON MICROSCOPE STUDY OF THE MITOCHONDRIAL STRUCTURE Journal of Histochemistry and Cytochemistry. ,vol. 1, pp. 188- 211 ,(1953) , 10.1177/1.4.188
Yongmei Chen, Raymond A. Swanson, Astrocytes and Brain Injury Journal of Cerebral Blood Flow and Metabolism. ,vol. 23, pp. 137- 149 ,(2003) , 10.1097/01.WCB.0000044631.80210.3C
Akio Takaoka, Toshiaki Hasegawa, Kiyokazu Yoshida, Hirotaro Mori, Microscopic tomography with ultra-HVEM and applications. Ultramicroscopy. ,vol. 108, pp. 230- 238 ,(2008) , 10.1016/J.ULTRAMIC.2007.06.008
Sibel Kahraman, Linda L. Bambrick, Gary Fiskum, Effects of FK506 and cyclosporin a on calcium ionophore-induced mitochondrial depolarization and cytosolic calcium in astrocytes and neurons Journal of Neuroscience Research. ,vol. 89, pp. 1973- 1978 ,(2011) , 10.1002/JNR.22709
Vincent Soubannier, Heidi M. McBride, Positioning mitochondrial plasticity within cellular signaling cascades. Biochimica et Biophysica Acta. ,vol. 1793, pp. 154- 170 ,(2009) , 10.1016/J.BBAMCR.2008.07.008
Kara L. Cerveny, Yasushi Tamura, Zhongyan Zhang, Robert E. Jensen, Hiromi Sesaki, Regulation of mitochondrial fusion and division. Trends in Cell Biology. ,vol. 17, pp. 563- 569 ,(2007) , 10.1016/J.TCB.2007.08.006