Interesterification of fat blends rich in ω-3 polyunsaturated fatty acids catalysed by immobilized Thermomyces lanuginosa lipase under high pressure

摘要: Abstract The interesterification of natural fats will improve certain physical and nutraceutical properties by modification their acylglycerol profile. In this study, the effect high pressure in kinetics fat blends, solvent-free medium, catalysed a commercial immobilized lipase from Thermomyces lanuginosa was evaluated. Reaction media were ternary blends palm stearin, kernel oil concentrate triacylglycerols (TAG) rich ω-3 polyunsaturated fatty acids. Reactions carried out at 60 °C, 0.1, 50, 100 150 MPa. followed (i) decrease “Solid Fat Content” values blend 35 °C (SFC35 °C) (ii) changes biocatalyst presented activity least up to Results obtained 0.1 MPa, with no agitation reaction found be similar those under high-pressure conditions. observed SFC35 °C accompanied important An increase compounds low equivalent carbon number (ECN) TAG ECN 44 46 observed. This consumption 48 50 for all values, also trilaurin (ECN = 36) normal pressure. High pressures seem affect selectivity towards lauric acid. Batch operational stability tests showed linear inactivation profile each Half-lives about 15, 6 4 h estimated 150 MPa, respectively.