DNA-Free Genome Editing of Brassica oleracea and B. rapa Protoplasts Using CRISPR-Cas9 Ribonucleoprotein Complexes
作者： Jana Murovec , Katja Guček , Borut Bohanec , Monika Avbelj , Roman Jerala
关键词:
摘要: … of Cas9:sgRNA could be drawn from the results as they differed substantially between loci. In the other reports about the use of RNPs (Cas9 + … about transfection of protoplasts with PEG. …
frontiersin.org
sci-hub.se 参考文章(30)
Saminathan Subburaj, Sung Jin Chung, Choongil Lee, Seuk-Min Ryu, Duk Hyoung Kim, Jin-Soo Kim, Sangsu Bae, Geung-Joo Lee, Site-directed mutagenesis in Petunia × hybrida protoplast system using direct delivery of purified recombinant Cas9 ribonucleoproteins. Plant Cell Reports. ,vol. 35, pp. 1535- 1544 ,(2016) , 10.1007/S00299-016-1937-7
Luca Pinello, Matthew C Canver, Megan D Hoban, Stuart H Orkin, Donald B Kohn, Daniel E Bauer, Guo-Cheng Yuan, Analyzing CRISPR genome-editing experiments with CRISPResso Nature Biotechnology. ,vol. 34, pp. 695- 697 ,(2016) , 10.1038/NBT.3583
Jeongbin Park, Kayeong Lim, Jin-Soo Kim, Sangsu Bae, Cas-analyzer: an online tool for assessing genome editing results using NGS data Bioinformatics. ,vol. 33, pp. 286- 288 ,(2017) , 10.1093/BIOINFORMATICS/BTW561
Sergei Svitashev, Christine Schwartz, Brian Lenderts, Joshua K. Young, A. Mark Cigan, Genome editing in maize directed by CRISPR–Cas9 ribonucleoprotein complexes Nature Communications. ,vol. 7, pp. 13274- 13274 ,(2016) , 10.1038/NCOMMS13274
Mickael Malnoy, Roberto Viola, Min-Hee Jung, Ok-Jae Koo, Seokjoong Kim, Jin-Soo Kim, Riccardo Velasco, Chidananda Nagamangala Kanchiswamy, DNA-Free Genetically Edited Grapevine and Apple Protoplast Using CRISPR/Cas9 Ribonucleoproteins Frontiers in Plant Science. ,vol. 7, pp. 1904- 1904 ,(2016) , 10.3389/FPLS.2016.01904
Zhen Liang, Kunling Chen, Tingdong Li, Yi Zhang, Yanpeng Wang, Qian Zhao, Jinxing Liu, Huawei Zhang, Cuimin Liu, Yidong Ran, Caixia Gao, Efficient DNA-free genome editing of bread wheat using CRISPR/Cas9 ribonucleoprotein complexes Nature Communications. ,vol. 8, pp. 14261- ,(2017) , 10.1038/NCOMMS14261
Hyeran Kim, Sang-Tae Kim, Jahee Ryu, Beum-Chang Kang, Jin-Soo Kim, Sang-Gyu Kim, CRISPR/Cpf1-mediated DNA-free plant genome editing Nature Communications. ,vol. 8, pp. 14406- 14406 ,(2017) , 10.1038/NCOMMS14406
Lei Kang, Pengfei Li, Aifan Wang, Xianhong Ge, Zaiyun Li, A Novel Cytoplasmic Male Sterility in Brassica napus (inap CMS) with Carpelloid Stamens via Protoplast Fusion with Chinese Woad. Frontiers in Plant Science. ,vol. 8, pp. 529- 529 ,(2017) , 10.3389/FPLS.2017.00529
Janina Braatz, Hans-Joachim Harloff, Martin Mascher, Nils Stein, Axel Himmelbach, Christian Jung, CRISPR-Cas9 Targeted Mutagenesis Leads to Simultaneous Modification of Different Homoeologous Gene Copies in Polyploid Oilseed Rape (Brassica napus). Plant Physiology. ,vol. 174, pp. 935- 942 ,(2017) , 10.1104/PP.17.00426
Hong Yang, Jia-Jing Wu, Ting Tang, Ke-De Liu, Cheng Dai, CRISPR/Cas9-mediated genome editing efficiently creates specific mutations at multiple loci using one sgRNA in Brassica napus Scientific Reports. ,vol. 7, pp. 7489- 7489 ,(2017) , 10.1038/S41598-017-07871-9