Effects of Glucose and Insulin on Rat Apolipoprotein A-I Gene Expression
作者： Koji Murao , Yoshinaru Wada , Takaaki Nakamura , Anthony H. Taylor , Arshag D. Mooradian
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摘要: Abstract We have examined the regulation of apolipoprotein A-I (apoA-I) gene expression in response to glucose and insulin. In Hep G2 cells, endogenous apoA-I mRNA was suppressed by one-half or induced 2-fold following 48 h exposure high concentrations (22.4 mm) insulin (100 microunits/ml), respectively, compared with control. Transcriptional activity rat promoter (−474 −7) cells paralleled expression, this dependent on dose Deletional analysis showed that a 50-base pair fragment spanning −425 −376 mediated effects both glucose. Within DNA there is motif (−411 −404) homologous previously identified core element (IRCE). Mutation abolished not only induction but also abrogated its suppression Electrophoretic mobility shift assay nuclear extracts from revealed IRCE binding formed duplex radiolabeled probe. The correlated expression. summary, our data show decreases increases activity. This effect appears be single cis-acting element.
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