A cytotoxic human hybridoma monoclonal antibody (TrJ6) defining an epitope expressed by HLA-DQ4 and -DQ5.
作者： Jun Ge , Kristian Hannestad
DOI: 10.1016/0198-8859(94)90108-2
关键词:
摘要: Abstract We have generated a cytotoxic human hybridoma monoclonal IgMλ antibody, designated TrJ6, that is specific for new epitope shared by HLA-DQ4 and -DQ5. TrJ6 strongly killed all ten DQ4-bearing cells weakly four DQ5-bearing cell lines. In contrast, none of the 36 lines lacking DQ4 DQ5 antigens was recognized TrJ6. This confirmed fluorescence cytometry. The binding to line efficiently blocked IIB3 (murine anti-DQ8 + 9 4 5 6 mAb) TrG6 (human IgG mAb against 6), confirming polymorphic DQ epitope. can be used distinguish from DQ6 B-lymphoblastoid cells. DQ4s DQ5s chains share one unique residue (Ser-74) relatively (Val-75), which may therefore need coexpressed in order formed. Alternatively, Ser-74 alone contributes critically allospecificity this addition, or more three residues (Leu-56, Glu-70, Asp-71 on chain) could also contribute because reacted stronger with DQ4- than
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