Water stress induces up-regulation of DOF1 and MIF1 transcription factors and down-regulation of proteins involved in secondary metabolism in amaranth roots (Amaranthus hypochondriacus L.).

摘要: Roots are the primary sites of water stress perception in plants. The aim this work was to study differential expression proteins and transcripts amaranth roots (Amaranthus hypochondriacus L.) when plants were grown under drought stress. Changes protein abundance within examined using two-dimensional electrophoresis LC/ESI-MS/MS, evaluated with suppression subtractive hybridisation (SSH). Induction decreased relative content leaves increased solutes such as proline total soluble sugars roots. Differentially expressed SOD(Cu-Zn) , heat shock proteins, signalling-related glycine-rich identified. Up-regulated those related defence, stress, signalling (Ser, Tyr-kinases phosphatases) transport (aquaporins nodulins). More noteworthy identification transcription factors DOF1, which has been several plant-specific biological processes, MIF1, whose constitutive root growth reduction dwarfism. down-regulated genes/proteins identified cell differentiation (WOX5A) secondary metabolism (caffeic acid O-methyltransferase, isoflavone reductase-like two different S-adenosylmethionine synthetases). Amaranth response appears involve a coordinated osmolyte accumulation, up-regulation that control damage from reactive oxygen species, family stabilise other plant control.