Lethal and sublethal effects of the pirimicarb-based formulation Aficida® on Boana pulchella (Duméril and Bibron, 1841) tadpoles (Anura, Hylidae)

摘要: Abstract Acute lethal and sublethal toxicity of the pirimicarb-based commercial formulation Aficida ® were evaluated on Boana pulchella tadpoles. Whereas mortality was used as end point for lethality, frequency micronuclei other nuclear abnormalities well alterations in erythroblasts circulating blood biomarkers genotoxicity cytotoxicity, respectively. Swimming, growth, developmental morphological also employed points. Results show that species is within 13th percentile distribution acute sensitivity to pirimicarb aquatic vertebrates. revealed values 23.78 101.45 mg/L LC50 96 h GS25 GS36 tadpoles, The most evident effects related with swimming activity NOEC LOEC 0.005–0.39 mg/L concentration range. induced DNA damage at chromosomal level by increasing abnormalities, i.e. , lobbed notched nuclei binucleated cells. Cellular cytotoxicity found after treatment. presence abdominal oedemas exposed organisms thus flotation response could be proposed a new sensitive exposure parameter. multiple assessment approach allowed complete understanding multi occurring pirimicarb, least B. .