Gender Preselection in Cattle with Intracytoplasmically Injected, Flow Cytometrically Sorted Sperm Heads
作者： Koh-ichi Hamano , Xihe Li , Xiao-qiao Qian , Katsutoshi Funauchi , Makoto Furudate
DOI: 10.1095/BIOLREPROD60.5.1194
关键词:
摘要: We investigated the development to blastocyst and subsequent live-offspring stages of in vitro-matured bovine oocytes intracytoplasmically injected with flow cytometrically sorted bull sperm heads. Bull heads, prepared by ultrasound sonication, were distinguished on basis their relative DNA contents using a cytometer/cell sorter modified for sorting sperm. By fluorescence situ hybridization, proportion confirmed as having Y specific fraction was 82%. Injection single heads (cultured 24 h) resulted 46.6% cleavage 6.9% rates. Embryo transfer 48 blastocysts (Days 7-8) recipients (one per recipient) 20.8% pregnancy normal live offspring production The birth 8 male 2 female calves represents an 80% sex preselection accuracy rate.
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