PCR – the polymerase chain reaction

摘要: DNA-based procedures are becoming increasingly common within the analytical laboratory where polymerase chain reaction (PCR) has become an indispensable technique. Developed in 1985 by Kary B. Mullis, PCR revolutionized way that deoxyribonucleic acid (DNA) could be copied. Mullis's invention allowed researchers to make millions of copies a selected DNA region hours. Today, can take minutes. widespread applications food, environmental, medical and forensic fields. This Technical Brief covers basics some its variations.