An on-site, highly specific immunosensor for Escherichia coli detection in field milk samples from mastitis-affected dairy cattle.

摘要: Bovine mastitis is the most economically important infectious disease in dairy industry, and Escherichia coli (E. coli) one of major causative pathogens. Rapid identification quantitative detection E. are great importance for bovine control milk quality monitoring. Capitalizing on dielectrophoresis interphase capacitance sensing, we have developed an immunosensor by modifying low cost commercial microelectrodes with specific antibody. The limit reaches as 775 cells/mL within a 15 s' response time, which can satisfy requirement on-site field diagnosis mastitis. To demonstrate sensor's specificity, tests against Staphylococcus aureus Streptococcus uberis samples performed showing negligible responses, selectivity calculated to be 3063: 1. Furthermore, simple pretreatment protocol testing raw milk, only involves incubation, centrifugation dilution steps. Then correct demonstrated both artificially inoculated infected samples. This corresponding advantages speed, sensitivity, operability, cost, make it highly promising pathogen