Soybean Seed Lectin Prevents the Accumulation of S-Adenosyl Methionine Synthetase And the S1 30S Ribosomal Protein in Bradyrhizobium japonicum Under C and N Starvation

作者: Julieta Pérez-Giménez , Julieta M. Covelli , M. Florencia López , M. Julia Althabegoiti , Mario Ferrer-Navarro

DOI: 10.1007/S00284-012-0180-X

关键词:

摘要: Soybean lectin (SBL) participates in the recognition between Bradyrhizobium japonicum and soybean although its role remains unknown. To search for changes proteome response to SBL, B. USDA 110 was incubated 12 h a C- N-free medium with or without SBL (10 μg ml −1), soluble protein profiles were compared. Two polypeptides, S-adenosyl-methionine synthetase (MetK) 30S ribosomal S1 (RpsA), found only fractions from rhizobia SBL. Transcript levels of metK rpsA not correlated polypeptide levels, indicating that there regulation at translation. In support this proposal, 5′ translation initiation-region mRNA contained folding elements as those involved other species. Disappearance MetK RpsA SBL-treated suggests might have attenuated nutritional stress japonicum.

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