Rapidly Transducing and Spatially Localized Magnetofection Using Peptide-Mediated Non-Viral Gene Delivery Based on Iron Oxide Nanoparticles

摘要: Non-viral delivery systems are generally of low efficiency, which limits their use in gene therapy and editing applications. We previously developed a technology termed glycosaminoglycan (GAG)-binding enhanced transduction (GET) to efficiently deliver variety cargos intracellularly; our system employs GAG-binding peptides, promote cell targeting, penetrating peptides (CPPs), enhance endocytotic internalization. Herein, we describe further modification by combining magnetic targeting with the GET technology. associated plasmid (p)DNA, iron oxide superparamagnetic nanoparticles (MNPs), allowing rapid targeted GET-mediated uptake application static fields NIH3T3 cells. This produced effective transfection levels (significantly higher than control) seconds minutes exposure localized two orders magnitude over non-targeted monolayers using (in 15 min delivering GFP reporter pDNA). More importantly, high membrane GET-DNA MNP co-complexes allowed enhancement uptake, meaning that nucleic acid cargo was rapidly internalized beyond complexes alone (GET-DNA). Magnetofection MNPs combined allows field-guided local vitro could facilitate focused for future regenerative disease-targeted therapies vivo.