Molecular Analysis of 11q13 Breakpoints in Multiple Myeloma

摘要: The t(11;14)(q13;q32) chromosomal translocation, which is the hallmark of mantle cell lymphoma (MCL), found in approximately 30% multiple myeloma (MM) tumors with a 14q32 translocation. Although overexpression cyclin D1 has been to be correlated MM lines carrying t(11;14), rearrangements BCL-1/cyclin regions frequently involved MCL rarely occur or primary tumors. To test whether specific 11q13 breakpoint clusters may MM, we investigated representative panel by means Southern blot analysis using probes derived from MM-associated breakpoints. this end, first cloned breakpoints and respective germ-line tumor U266 line, as well region KMS-12 line. DNA 50 was tested large probes, but rearrangement detected only one case probe. Our results confirm previous findings that are scattered throughout encompassing gene, thus suggesting absence MM.