Proteomics analysis of contact-initiated eph receptor-ephrin signaling.
作者： Claus Jorgensen , Alexei Poliakov
DOI: 10.1007/978-1-62703-604-7_1
关键词:
摘要: Large-scale biochemical analysis of cell-specific signaling can be interrogated in cocultures Eph receptor- and ephrin-expressing cells by combining proteomics with metabolic labeling. In this chapter, we describe how to perform such large-scale analysis, including the generation stably expressing receptors ligands interest, optimization steps for Eph-ephrin coculture, analysis. As experimental details may vary depending on specific system that is being interrogated, goal chapter mainly provide sufficient context experienced researchers set up conduct these experiments.
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sci-hub.st 参考文章(27)
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