Histone IIb1 from rainbow trout. Comparison in amino acid sequence with calf thymus IIB1.

摘要: Histone IIb1 from immature testes of rainbow trout has been highly purified and then succinylated or maleylated treated with trypsin. Thirteen tryptic peptides have purified, a total composition accounting for all amino acid residues in intact IIb1. Eleven these sequenced completely, partial sequences obtained the other two peptides. Intact histone was also chymotrypsin N-bromosuccinimide. were isolated pure nearly form. Compositional sequence information allowed but to be placed unambiguous order, by homology recently published calf (Y eoman , L. C., O lson J. O. J., S ugano N., J ordan T aylor C. W., tarbuck W. B usch H. (1972) Biol. Chem. 247, 6018–6023). Rainbow appear differ primary four point substitutions deletions. The NH2-terminal half is conserved sequence, most striking evolutionary changes seen near COOH terminus molecule. regular spacing basic as well existence partially acetylated phosphorylated region suggests specific role this molecule interaction each phosphate backbones forming major groove DNA double helix eukaryotic chromosomes.