作者: G. Schmer , G. Kreil
DOI: 10.1016/0003-2697(69)90301-7
关键词:
摘要: Abstract A micro method for the detection of N -formyl and -acetyl groups in polypeptides is described. This based on following principle: acylhydrazine obtained after cleavage with anhydrous hydrazine incubated dansyl chloride. The reaction product, a 1-acyl-2-dansyl-hydrazine, can be identified by two-dimensional thin-layer chromatography. has been tested number peptides proteins known to contain formyl or acetyl at amino end.