Detection and quantitation of full-length thioredoxin (TRX) and truncated thioredoxin (TRX 80) in complex samples
作者： Susan Christine Trow , David Raymond Graham , Mary Christine Zink
DOI:
关键词:
摘要: The present invention relates, e.g., to a method for detecting full-length protein and truncated form (e.g., naturally occurring cleavage product) thereof, in sample, comprising optionally denaturing reducing proteins the cleaving into smaller peptides, unique peptide identifier and/or protein, sample. In one embodiment of invention, is thioredoxin (TRX), thereof its biologically active, C-terminal truncated, 10 kDa product, TRX 80.
lens.org 参考文章(53)
Jonathan A. Green, Margaret M. Manson, Production of polyclonal antisera. Methods of Molecular Biology. ,vol. 80, pp. 1- 5 ,(1992) , 10.1385/0-89603-204-3:1
G Köhler, C Milstein, Continuous cultures of fused cells secreting antibody of predefined specificity. 1975. Journal of Immunology. ,vol. 174, pp. 2453- 2455 ,(2005)
Takashi Okamoto, Toshifumi Tetsuka, Nobuo Matsui, Tetsuji Katoh, Kazuko Uno, Shinichi Yoshida, Involvement of Thioredoxin in Rheumatoid Arthritis: Its Costimulatory Roles in the TNF-α-Induced Production of IL-6 and IL-8 from Cultured Synovial Fibroblasts Journal of Immunology. ,vol. 163, pp. 351- 358 ,(1999)
J R David, S L Baker, M H Ali, D S Silberstein, Human eosinophil cytotoxicity-enhancing factor. Purification, physical characteristics, and partial amino acid sequence of an active polypeptide. Journal of Immunology. ,vol. 143, pp. 979- 983 ,(1989)
Katja Becker, Leann Tilley, Jonathan L. Vennerstrom, David Roberts, Stephen Rogerson, Hagai Ginsburg, Oxidative stress in malaria parasite-infected erythrocytes: Host-parasite interactions International Journal for Parasitology. ,vol. 34, pp. 163- 189 ,(2004) , 10.1016/J.IJPARA.2003.09.011
Amanda F. Baker, Tomislav Dragovich, Wendy R. Tate, Ramesh K. Ramanathan, Denise Roe, Chiu-Hsieh Hsu, D. Lynn Kirkpatrick, Garth Powis, The antitumor thioredoxin-1 inhibitor PX-12 (1-methylpropyl 2-imidazolyl disulfide) decreases thioredoxin-1 and VEGF levels in cancer patient plasma. Journal of Laboratory and Clinical Medicine. ,vol. 147, pp. 83- 90 ,(2006) , 10.1016/J.LAB.2005.09.001
Takumi Jikimoto, Yuko Nishikubo, Masahiro Koshiba, Sugayo Kanagawa, Sahoko Morinobu, Akio Morinobu, Ryuichi Saura, Kosaku Mizuno, Shohei Kondo, Shinya Toyokuni, Hajime Nakamura, Junji Yodoi, Shunichi Kumagai, Thioredoxin as a biomarker for oxidative stress in patients with rheumatoid arthritis. Molecular Immunology. ,vol. 38, pp. 765- 772 ,(2002) , 10.1016/S0161-5890(01)00113-4
Anne Burke-Gaffney, Matthew E.J. Callister, Hajime Nakamura, Thioredoxin: friend or foe in human disease? Trends in Pharmacological Sciences. ,vol. 26, pp. 398- 404 ,(2005) , 10.1016/J.TIPS.2005.06.005
M Christine Zink, Jennifer Uhrlaub, Jesse DeWitt, Tauni Voelker, Brandon Bullock, Joseph Mankowski, Patrick Tarwater, Janice Clements, Sheila Barber, Neuroprotective and anti-human immunodeficiency virus activity of minocycline JAMA. ,vol. 293, pp. 2003- 2011 ,(2005) , 10.1001/JAMA.293.16.2003
T. Fortin, A. Salvador, J. P. Charrier, C. Lenz, F. Bettsworth, X. Lacoux, G. Choquet-Kastylevsky, J. Lemoine, Multiple reaction monitoring cubed for protein quantification at the low nanogram/milliliter level in nondepleted human serum. Analytical Chemistry. ,vol. 81, pp. 9343- 9352 ,(2009) , 10.1021/AC901447H