Non-biological synthetic spike-in controls and the AMPtk software pipeline improve mycobiome data

作者: Jonathan M. Palmer , Michelle A. Jusino , Mark T. Banik , Daniel L. Lindner

DOI: 10.7717/PEERJ.4925

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摘要: … This was accomplished by PCR amplifying each individual plasmid with the same barcoded primer set. PCR products were purified using E-gel ® CloneWell™ 0.8% SYBR ® Safe …

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