Whole-brain calcium imaging during physiological vestibular stimulation in larval zebrafish
作者： Geoffrey Migault , Thomas Panier , Raphaël Candelier , Georges Debrégeas , Volker Bormuth
DOI: 10.1101/300350
关键词:
摘要: During in vivo functional imaging, animals are head-fixed and thus deprived from vestibular inputs, which severely hampers the design of naturalistic virtual environments. To overcome this limitation, we developed a miniaturized ultra-stable light-sheet microscope that can be dynamically rotated during imaging along with head-restrained zebrafish larva. We demonstrate system enables whole-brain at single-cell resolution under controlled stimulation. recorded for first time dynamic response vertebrate to physiological This development largely expands potential virtual-reality systems explore complex multisensory-motor integration 3D.
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