Sox9 transactivation and testicular expression of a novel human gene,KIAA0800

摘要: The Sry and Sox9 sex-determination factors initiate promote testis differentiation by gene transactivation through similar promoter elements. However, knowledge is limited concerning what genes are regulated Sry/Sox9 in the testis. Identification characterization of Sry/Sox9-regulated critical for understanding sexual differentiation. We now demonstrate that a novel human gene, KIAA0800, preferentially expressed transactivated Sox9. KIAA0800 repressed an upstream element involving polyT track two Alu repeats. Two specific Sox9-bindings sites have been identified using DNaseI footprinting assays gel electrophoretic mobility shift assays. appears to be exerted mainly relief repression. Genes homologous exist organisms with differentiated sex tissues including mouse, Drosophila, C. elegans, but not unicellular organisms, yeast bacteria. Further, our recent sequence analysis shows protein 97% identical between mouse. Thus, Sox9-activated evolutionarily conserved potentially involved J. Cell. Biochem. 86: 277–289, 2002. © 2002 Wiley-Liss, Inc.