Implementation of design of experiments for optimization of forced degradation conditions and development of a stability-indicating method for furosemide.

摘要: The study involved optimization of forced degradation conditions and development a stability-indicating method (SIM) for furosemide employing the design experiment (DoE) concept. conditions, especially hydrolytic oxidative, was done by application 2(n) full factorial designs, which helped to obtain targeted 20-30% drug also enriched levels products (DPs). For selective separation its DPs SIM, DoE applied in three different stages, i.e., primary parameter selection, secondary screening optimization. these three, IV-optimal, Taguchi orthogonal array face-centred central composite designs were employed, respectively. organic modifier, buffer pH, gradient time initial hold selected as parameters. Initial final modifier percentage, flow rate came out critical parameters during screening, further evaluated Based on results, an optimized obtained wherein total twelve separated successfully. exposed behaviour conditions.