Multi-step optimization of the filtration method for the isolation of Campylobacter species from stool samples

摘要: The filtration method (FM) is the most effective isolation technique for Epsilobacteriaceae from stool samples. FM’s different adaptations make it difficult to compare data between studies. This study was performed in three phases optimize FM a routine laboratory perspective. In July–September 2014 (part I), on Mueller–Hinton agar containing 5% sheep blood and Columbia blood. July 2016 II), using 0.60-μm pore size polycarbonate filters (0.6-PC filter) 0.45-μm cellulose acetate (0.45-AC filter); January 2018 III), addition of hydrogen incubators studied. On 1146 stools analyzed part I, positive samples that showed no growth Butzler medium (n = 22/72, 30.6%) had improved when instead (21/22 strains vs. 11/22, p < 0.05). II, 718 stools, 91 grew with (12.7%), more 0.6-PC filter (90/91) than 0.45-AC (44/91) (p < 0.05). III, 578 were cultured, 98 FM, 7% finding significantly without (90/98, 91.8%, 72/98, 73.5%; use incubated at 37 °C hydrogen-enriched atmosphere led an almost fourfold increase rate among studied combinations. Reference centers Campylobacter should standardized protocols enable comparison prevalence space time.