Processing and ligand-induced modifications of the V2 vasopressin receptor.

摘要: Synthesis, processing and agonist-induced modifications of the V2 vasopressin receptor were examined in stably or transiently transfected HEK293 cells. Metabolic labeling with35S methionine for 30 min revealed a predominant precursor protein which subsequently gave rise to mature on cell surface. Maturation was unrelated glycosylation suggesting that it consequence refolding. In addition monomeric forms protein, oligomers also detected SDS-PAGE. These seemed be dimers tetrameres, more apparent cells produced higher quantities then No detected, co-transfection wild type with mutant lacking G-protein coupling activity did not alter function receptor. results indicated formation oligomeric most likely overproduction required step function.