Noscapine protects the H9c2 cardiomyocytes of rats against oxygen-glucose deprivation/reperfusion injury.

摘要: Noscapine is an antitumor alkaloid derived from Papaver somniferum plants. Our previous study has demonstrated that exposure of noscapine on primary murine fetal cortical neurons exposed to oxygen–glucose deprivation/reperfusion (OGD/R) neuroprotective effects. In current study, the effects cardiomyocytes (H9c2 cells) damage caused by 120 minutes (min) OGD/R were evaluated and we determined whether addition BD1047, sigma-one receptor antagonist, prevents protective in H9c2 cells through production nitric oxide (NO) apoptosis. To initiate OGD, was transferred glucose-free DMEM, placed a humidified incubation chamber. Cell viability assessed with (1–5 μM) presence or absence 24 hours (h) after OGD/R. viability, NO apoptosis ratio MTT assay, Griess method quantitative real-time PCR. considerably improved survival compared Also, extremely capable reducing concentrations Bax/Bcl-2 expression. While BD1047 administration alone diminished cell increased levels. The did not increase relative alone. exerted cardioprotective OGD/R-induced injury cells, at least partly via attenuation ratio, which indicates activation involved protection injured