Characterization of an Krox-24/Egr-1-responsive Element in the Human Tumor Necrosis Factor Promoter
作者： Bernd Krämer , Albrecht Meichle , Gabriele Hensel , Patrick Charnay , Martin Krönke
DOI: 10.1016/0167-4781(94)90066-3
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摘要: Abstract We have analyzed in various human leukemic cell lines a previously unrecognized region within the TNF gene promoter that contains sequence motif 5′-CCGCCCCCGCG-3′. This GC-rich maps to bps −170 and −160 of gene. Electrophoretic mobility shift assays (EMSA) combined with methylation interference analysis revealed binding two distinct proteins overlapping recognition sites. Supershift identified constitutive transcription factor Sp1 immediate-early growth-response Egr-1/Krox-24. Interestingly, this Egr-1-related was induced by PMA but not TNF. The conferred responsiveness when linked heterologous minimal c-fos promoter. To examine involvement Egr-1/Krox-24 regulation, Krox-24 expression vector used, pSCTKr24. In Jurkat T cells pSCTKr24 stimulated pTNF-286CAT sequences −286 +34 fused chloramphenicol acetyltransferase (CAT) Moreover, also However, deletion site did result markedly reduced activity, suggesting response element may play an auxiliary role regulation.
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