Grapevine powdery mildew resistance and susceptibility loci identified on a high-resolution SNP map

摘要: Improved efficacy and durability of powdery mildew resistance can be enhanced via knowledge the genetics susceptibility coupled with development high-resolution maps to facilitate stacking multiple genes other desirable traits. We studied inheritance (Erysiphe necator) wild Vitis rupestris B38 cultivated V. vinifera ‘Chardonnay’, finding evidence for quantitative variation. Molecular markers were identified using genotyping-by-sequencing, resulting in 16,833 single nucleotide polymorphisms (SNPs) based on alignment ‘PN40024’ reference genome sequence. With an average density 36 SNPs/Mbp uniform coverage genome, this 17K set was used identify 11 SNPs chromosome 7 associated a locus from ten 9 ‘Chardonnay’ marker association linkage disequilibrium analysis. Linkage (1,146 SNPs) (1,215 constructed corroborate named Sen1 (Susceptibility Erysiphe necator 1), providing first insight into vinifera. The identification background negative selection among breeding progenies. This work improves our understanding nature while applying next-generation sequencing tools advance grapevine genomics breeding.