Gene expression profiling of microdissected pancreatic ductal carcinomas using high-density DNA microarrays.
作者： Robert Grützmann , Christian Pilarsky , Ole Ammerpohl , Jutta Luttges , Armin Böhme
DOI: 10.1593/NEO.04295
关键词:
摘要: Pancreatic ductal adenocarcinoma (PDAC) remains an important cause of malignancy-related death and is the eighth most common cancer with lowest overall 5-year relative survival rate. To identify new molecular markers candidates for therapeutic regimens, we investigated gene expression profile microdissected cells from 11 normal pancreatic ducts, 14 samples PDAC, 4 well-characterized cell lines using Affymetrix U133 GeneChip set. RNA was extracted lines, amplified, labeled a repetitive in vitro transcription protocol. Differentially expressed genes were identified significance analysis microarrays program. We found 616 differentially genes. Within these, 140 also PDAC by others, such as Galectin-1, Galectin-3, MT-SP2. validated differential several (e.g., CENPF, MCM2, MCM7, RAMP, IRAK1, PTTG1) immunohistochemistry reverse polymerase chain reaction. present whole genome study tissues highdensity microarrays. panel genes, novel which have not been associated pathogenesis before.
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