Distribution of the 22 cytokine gene polymorphisms in healthy Macedonian population.

摘要: BACKGROUND Distribution of cytokine gene polymorphisms may vary significantly among different ethnic groups, and eventually contribute to observed differences in disease frequencies. OBJECTIVES To genotype 22 the Macedonian population. The population consists 301 healthy unrelated individuals. METHODS Blood samples were collected after written consent, DNA was isolated from peripheral blood, typed: IL-1alpha -889, IL-1beta -511, +3962, IL-1R psti1970, IL-1RN mspa11100, IL-4Ralpha +1902, IL-12 -1188, IFNgamma utr5644, TGF-beta1 cdn10, cdn25, TNF-alpha -308, -238, IL-2 -330, +166, IL-4 -1098, -590, -33, IL-6 -174, 565, IL-10 -1082, -819, -592. Cytokine genotyping performed by PCR-SSP (Heidelberg kit). genetics analysis package, PyPop, used for data. RESULTS Test neutrality (Fnd) showed negative value, but 0 1 cdn10 utr5644 (p F = 0.001, 0.012 respectively). Several SNPs (IL-1alpha + 166, -592) not HWP 0.005). haplotypes (TGF-beta1, TNFalpha, IL-2, IL-4, IL-6, IL-10) difference only (p=0.020). CONCLUSION results can be anthropological comparisons, as well association studies with diseases (Tab. 6, Ref. 34). Full Text (Free, PDF) www. bmj. sk.