Immunosuppressor activity of rat endometrial granulated cells and their differentiation

摘要: Natural killer and natural suppressor activities of the rat endometrial granulated cells were assayed on days 13 14 pregnancy or pseudopregnancy. Metrial gland used as cells. The metrial other determined by means Hashimoto-Sudo test with K562 targets. estimation activity included removal sticking to glass from a suspension material Cytochemically, identified presence PAS-positive granules in cytoplasm after treatment diastase identification specific antigen help antisera. was twice weaker than that splenocytes same pregnant pseudopregnant females. level proportional content cell system. These data suggest is realized via their contact simultaneously for common When estimating cells, an effector test. Various amounts added effector: target system at ratio 50 : 1. did not depend amount present After fractionation Percoll gradient, highest recorded 60% fraction. lowest 30% fractions, respectively. culture medium characterized well. differences mean areas 30 fractions between (144.7 ± 13.4 75.0 12.5 µm2, respectively) (97.5 4.9 69.2 3.5 females reliable. estimated all studied 23 samples among which 18 (79.6 7.8%) displayed absence five combined this reduction area fraction 109 5 µm2. obtained confirm known low demonstrated first time these It concluded due differentiation activity.