Root-associated microbial communities of different strawberry cultivars as influenced by soil type, Verticillium dahliae Kleb. and biofumigation

摘要: Rhizosphere microorganisms and their interactions with plant roots play pivotal roles in controlling nutrition health. Extremely high levels of soil microbial diversity, coupled low cultivability, complicate the study these organisms but better mechanistic understanding each other is a prerequisite for development sustainable management strategies to improve nutrient acquisition control pathogens. This thesis describes different experiments designed investigate how community structure fungi associated rhizosphere soils strawberry plants are influenced by types, cultivars, presence or absence soil-borne fungal pathogen Verticillium dahliae biofumigation using residues oilseed radish Raphanus sativus oleifera. In an outdoor pot experiment, cloning Sanger sequencing, composition overall colonization arbuscular mycorrhizal appeared be more strongly type than cultivars. In similar experiment effects inoculation on total were analyzed throughput 454-pyrosequencing. The V. resulted significant reduction numbers operational taxonomic units (OTUs) four cultivars grown conventionally managed soil, organically no two large increase OTUs Florence, tolerant cultivar. Non-metric multidimensional scaling (NMDS) analysis communities less diverse peat-based revealed distinct clusters non-Verticillium treatments this effect was not visible field soils. A third 454 pyrosequencing, indicated significantly increased Verticillium-inoculation plants, suggesting green-manuring incorporation. Biofumigation did affect NMDS showed clear all structure. Complementary analyses changes bacterial same progress will hopefully shed light possible functional underlying treatment enable construction hypotheses that can tested further experiments.