Adsorption of β-lactoglobulin onto silica, methylated silica, and polysulfone

摘要: Milk and whey are widely processed by membrane filtration, often using polysulphone membranes. Adsorption of β-lactoglobulin onto was studied at protein concn. 0.1 1.0%, as well 12% to represent encountered during ultrafiltration. silica methylated surfaces (representing strongly hydrophilic hydrophobic resp.) also studied. Protein dissolved in 0.01 smallcap˜M phosphate buffer pH 7.0 containing 0.15 NaC1 adsorption/desorption monitored situ a Rudolph Thin Film ellipsometer. Polysulphone adsorbed the greatest amount least; intermediate. Differences between may reflect differences surface roughness. not reversed on dilution, whereas adsorption partially reversible. Pretreatment with 0.1% markedly reduced subsequent from (equivalent solution alone); preadsorption had much less effect adsorption. Methylated concluded be representative model for surface. (Less)