Positional specifity of acetylxylan esterases on natural polysaccharide: an NMR study.

摘要: Abstract Background Microbial degradation of acetylated plant hemicelluloses involves besides enzymes cleaving the glycosidic linkages also deacetylating enzymes. A detailed knowledge mode action these is important in view development efficient bioconversion materials that did not undergo alkaline pretreatment leading to hydrolysis ester linkages. Methods In this work deacetylation hardwood acetylglucuronoxylan by acetylxylan esterases from Streptomyces lividans (carbohydrate esterase family 4) and Orpinomyces sp. 6) was monitored 1 H-NMR spectroscopy. Results The resonances all acetyl groups polysaccharide were fully assigned. targets both are 2- 3-monoacetylated xylopyranosyl residues and, case enzyme, 2,3-di- O -acetylated residues. Both do recognize as a substrate 3- -acetyl group on α-1,2-substituted with 4- -methyl- d -glucuronic acid. Conclusions spectroscopy approach study positional specificity AcXEs outlined appears be simple way characterize catalytic properties belonging various CE families. Significance results contribute environmentally friendly procedures for enzymatic biomass.