An ORFeome-based Analysis of Human Transcription Factor Genes and the Construction of a Microarray to Interrogate Their Expression

摘要: Transcription factors (TFs) are essential regulators of gene expression, and mutated TF genes have been shown to cause numerous human genetic diseases. Yet date, no single, comprehensive database TFs exists. In this work, we describe the collection an essentially complete set from one depiction ORFeome, design a microarray interrogate their expression. Taking 1468 known TRANSFAC, InterPro, FlyBase, used seed search ScriptSure transcriptome for additional genes. ScriptSure's genome-anchored transcript clusters allowed us work with nonredundant high-quality representation transcriptome. We high-stringency similarity by using BLASTN, protein motif ORFeome hidden Markov models DNA-binding domains occur exclusively or primarily in TFs. Four hundred ninety-four were identified overlap between two searches, bringing our estimate total number 1962. Zinc finger far most abundant family (762 members), followed homeobox (199 members) basic helix-loop-helix (117 members). designed 50-mer oligonucleotide probes targeted unique region coding sequence each gene. successfully expression species as diverse chickens mice, well humans.