Isolation of microsatellite primers for Melampyrum sylvaticum (Orobanchaceae), an endangered plant in the United Kingdom

摘要:  Premise of the study: Microsatellite markers were developed for hemiparasitic plant Melampyrum sylvaticum to investigate breeding system, genetic diversity, and structure populations in United Kingdom, Sweden, Norway. Methods Results: Microsatellites isolated from genomic DNA using an enrichment protocol. Twenty-nine loci characterized two individuals each 15 geographically disparate (“global”). Seven polymorphic further one population (“local”). The number alleles per locus ranged 12 global sample seven local sample. expected heterozygosity 0–0.75, observed 0–0.1, inbreeding coeffi cient 0.84–1 Conclusions: results show utility these novel microsatellite conservation analyses. strong defi cit across all suggests species may be inbreeding.